Journal: Frontiers in Veterinary Science

Article Title: Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response

doi: 10.3389/fvets.2022.917389

Figure Lengend Snippet: Monoclonal and polyclonal antibodies (mAbs) used in this study for flow cytometry analysis and immunological assay.

Article Snippet: Anti-TNF alpha , Alexa Fluor® 488 , CC327 , Mouse , 1:200 , MCA2334A488 (AbD Serotec, United Kingdom).

Techniques: Flow Cytometry

Journal: Pediatric Research

Article Title: Lipopolysaccharide Preconditioning Reduces Neuroinflammation Against Hypoxic Ischemia and Provides Long-Term Outcome of Neuroprotection in Neonatal Rat

doi: 10.1203/pdr.0b013e3181b0d336

Figure Lengend Snippet: Figure 1. Immunohistochemistry for CD-11b and TNF-alpha per- formed 12 h after LPS injections. LPS activated few microglias in the cerebral cortex (A) 12-h postinjec- tion; however, there were signifi- cantly more CD-11b () cells (ar- rows) in the white matter (B) of rats treated with LPS0.3 than those treated with LPS0.05 or NS. TNF- alpha levels were significantly higher in the cerebral cortex (C) and white matter (D) in the LPS0.3 group than in the other two groups (IOD, inte- grated OD). Differences in TNF- alpha levels between the LPS0.05 and NS groups were not significant. Scale bar 50 m in (A, B, C) and 100 m in (D). Data are from three rats in each group. Values are means SEM. *p 0.05, and **p 0.01.

Article Snippet: The specific primary antibodies included anti-rat TNF-alpha antibody (1:100) (Cat. # BMS175; Bender MedSystems, Vienna, Austria) and anti-rat microglia and macrophage antibody (CD-11b; 1:100) (Cat. # MCA275R; AbD Serotec, Oxford, UK).

Techniques: Immunohistochemistry

Journal: Pediatric Research

Article Title: Lipopolysaccharide Preconditioning Reduces Neuroinflammation Against Hypoxic Ischemia and Provides Long-Term Outcome of Neuroprotection in Neonatal Rat

doi: 10.1203/pdr.0b013e3181b0d336

Figure Lengend Snippet: Figure 3. Immunohistochemistry for CD-11b and TNF-alpha per- formed 24 h after HI. The LPS-HI group had significantly reduced CD- 11b-() cells in the ipsilateral cortex (A) and white matter (B) than the NS-HI group. TNF-alpha level was also significantly decreased in the ce- rebral cortex (C) and white matter (D) in the LPS-HI group than in the NS-HI group. Data are from three rats in each group. Values are means SEM. Scale bar 50 m in (A-D). *p 0.05, **p 0.01, and §p 0.001.

Article Snippet: The specific primary antibodies included anti-rat TNF-alpha antibody (1:100) (Cat. # BMS175; Bender MedSystems, Vienna, Austria) and anti-rat microglia and macrophage antibody (CD-11b; 1:100) (Cat. # MCA275R; AbD Serotec, Oxford, UK).

Techniques: Immunohistochemistry